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You were recently infected and tested before your body produced Lyme antibodies.
You took antibiotics before testing which co-opted the antibody response
You were already on long term antibiotics for another illness
No enough “free” Lyme antibodies were detectable in your blood because they were all doing their job binding to the Lyme bacteria
Lyme spirochetes were protected and hiding inside a biofilm colony
Spirochetes were burrowed deep inside your body (i.e. cartilage, fibroblasts, neurons.etc)
Only small blebs were in your body, no whole bacteria which are needed for the PCR (polymerase chain reaction) based tests.
No free spirochetes in body fluid on day of test
Genetic heterogeneity (there are at least 300 strains of Lyme, 100 in the U.S). You might be infected by a strain of Borrelia that the test doesn’t recognize.
Antigen variability: Borrelia can change its outer surface protein to suit its environment so the test will detect a “non-Lyme specific” antibody
Spirochetes are in dormancy phase (L-form) with no cell walls so there is nothing for the immune system to attack with antibodies.
Lyme’s surface antigens can change body temperature
You have an immune deficiency (body isn’t producing antibodies)
You have had a recent anti-inflammatory treatment which suppress the immune system. (i.e., steroids, arthritis meds)
Co-infections with Babesia (protozoa) which causes immune suppression
Down-regulation of your immune system by your body’s own cytokines
Lab error or poor technical capability/training to detect Lyme.
You might have late stage Lyme. Lab test are not standardized for detecting late stage Lyme.
THe lab tests might only have been approved for investigational use
Lack of adequate reference points for the test (most tests only use a few genetic strains as reference)
The revised Western Blot criteria fails to include important antibody bands (i.e., 31,34)
CDC testing criteria is designed for epidemiological study, not clinical diagnostics.
* Not valid with Medicare or Medicaid
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